Applied these two technology together, more application will be found in biological research. Highspeed fourier ptychographic microscopy based on. The capability of bypassing the diffraction limit has made structured illumination technique a popular tool for subdiffraction imaging, enabling microscopy with high spatiotemporal resolution and reduced phototoxicity. Fourier ptychography microscopy fpm is a recently emerged. Click download or read online button to get fourier ptychographic imaging book now.
A wide field, highresolution imaging scheme that offers enhanced depth of field is demonstrated. Fourier ptychographic microscopy fpm is a highresolution computational imaging technique that is robust to recover highresolution image and large sbp by anglevaried illumination 4,5,6. In other words, is it possible to accurately recover an hr image with large fov. Wide field highresolution 3d microscopy with fourier ptychographic diffraction tomography article pdf available in optics and lasers in engineering 1285. In this article, we report an imaging method, termed fourier ptychographic microscopy fpm, which iteratively stitches together a number of variably illuminated, lowresolution intensity images in fourier space to produce a wide field, highresolution complex sample image.
In comparison with the digital pathology slide scanner, the fpm technique needs no mechanical scanning. Cover paper shaowei jiang, jiakai zhu, pengming song, chengfei guo, zichao bian, ruihai wang, yikun huang, shiyao wang, he zhang, and guoan zheng, wide field, highresolution lensless onchip microscopy via nearfield blind ptychographic modulation, lab on a chip, 2020, doi. Chen, wide field highresolution 3d microscopy with fourier ptychographic diffraction tomography, arxiv preprint arxiv. In other words, conventional microscopes compromise between a wide field of. Resolutionenhanced fourier ptychographic microscopy. Fourier ptychographic microscopy fpm, shares its roots with interferometric synthetic aperture microscopy720, ptychography2127, phase retrieval2831, light. Fourier ptychography fp 1 is an effective imaging technique that aims to tackle the physical limitation by capturing a sequence of sbp limited images and computationally combining them to recover a high resolution, large fov image. Pdf diffraction tomography with fourier ptychography. However, the authors believe that with either coherent or incoherent. Observing the various anatomical and functional information that spans many spatiotemporal scales with high resolution provides deep understandings of the fundamentals of biological systems.
Pdf we present an advanced lowcost widefield, highresolution computational microscope kit based on fourier ptychography. We report a computational 3d microscopy technique, termed fourier ptychographic diffraction tomography fpdt, that iteratively stitches together numerous variably illuminated, lowresolution images acquired with a lownumerical aperture na objective in 3d fourier space to create a wide fieldofview fov, highresolution, depthresolved complex refractive index ri image across. As such, fpm is able to bypass the design conflicts of conventional microscopes and achieve highresolution, wide fieldofview imaging capabilities. The approach relies on stitching together timemultiplexed images in fourier space. Osa fast and robust misalignment correction of fourier. The unique feature of the fpm is its capability to reconstruct images with both. Widefield fourier ptychographic microscopy using laser illumination source. Total internal reflection fourier ptychographic refractive. Osa neural network model combined with pupil recovery. In this paper, we report a wide fov microscopy system that uses a closedcircuittelevision cctv lens for image relay and a.
Fourier ptychographic microscopy fpm is a recently developed imaging approach aiming at circumventing the limitation of the spacebandwidth product sbp and acquiring a complex image with both wide field and high resolution. The unique feature of the fpm is its capability to reconstruct images with both wide. We report an imaging method, termed fourier ptychographic microscopy fpm, which iteratively stitches together a\ud number of variably illuminated, lowresolution intensity images in fourier space to produce a wide field, highresolution\ud complex sample image. Mafpm configuration utilises an array of objective lenses coupled with detectors to. Fourier ptychographic microscopy fpm is a new computational imaging technique that can provide gigapixel images with both a highresolution and a wide field of view. It is inherently difficult for a conventional microscopic imaging system to produce images with both wide field of view fov and highresolution because of its limited space bandwidth product. Widefield, highresolution lensless onchip microscopy. Pdf sampling criteria for fourier ptychographic microscopy in. Digital refocusing and extended depth of field reconstruction. Lowcost, submicron resolution, widefield computational.
Widefield, highresolution fourier ptychographic microscopy. Among different approaches, fourier ptychographic microscopy fpm is probably the most promising technique to realize highthroughput qpi by synthesizing a wide. Fourier ptychographic microscopy fpm is a new computational imaging technique that circumvents this limit by fusing information from multiple images taken with different illumination angles. The only required hardware is a standard microscope and an array of leds. To increase temporal resolution, one may use multiplexed measurements where the sample is illuminated. Aug 27, 2015 in the version of this article originally published, the reported resolution for the microscope was the halfpitch resolution.
Lightfield microscopy lfm has recently emerged as a scanningfree, scalable method that allows for highspeed, volumetric imaging ranging from singlecell specimens to the mammalian brain. Apr 01, 2016 fourier ptychographic microscopy fpm is a newly developed super resolution technique, which employs angularly varying illuminations and a phase retrieval algorithm to surpass the diffraction limit of a low numerical aperture na objective lens. The capability to perform highresolution, wide fieldofview fov microscopy imaging is highly sought after in biomedical applications. Wo2015179452a1 fourier ptychographic microscopy with. Guoan zheng, roarke horstmeyer, changhuei yang download pdf. Waller, 3d intensity and phase imaging from light field measurements in an led array microscope. Jul 28, 20 a wide field, highresolution imaging scheme that offers enhanced depth of field is demonstrated. Counting white blood cells from a blood smear using fourier. Guoan zheng, xiaoze ou, roarke horstmeyer, jaebum chung and changhuei yang. In this paper, a novel microscopic imaging technology, termed light field fourier ptychographic microscopy lffpm, is presented which enables the reconstruction of wide field, highresolution images with the depth information by utilizing a lightemittingdiode led illumination matrix.
Adaptive denoising method for fourier ptychographic microscopy yao fan a,b c, jiasong sun, qian chen a,b, mingqun wang c, chao zuoa,b c a school of electronic and optical engineering, nanjing university of science and technology, no. Guoan zheng, roarke horstmeyer, and changhuei yang. Automatic methods are capable of counting a large number of cells to give a statistically more accurate reading of the wbc count of a sample, but the specialized. Meanwhile, a wide field, highresolution imaging technique termed fourier ptychographic microscopy fpm,,,,,, has seen significant advancements since it was first proposed and demonstrated by guoan zheng. A gigapixel superscope for biomedicine guoan zheng, xiaoze ou, roarke horstmeyer, jaebum chung and changhuei yang a simple modification, combined with computational optics, can boost the resolving power of standard microscopes by more than an order of magnitudeand open up new applications in both the lab and. The capacity of fpm for high resolution imaging at low magnification makes it particularly attractive for applications in. The individual lights in the led array can be actuated according to random, nonrandom and hybrid random and nonrandom illumination strategies to produce high resolution images with fast acquisition speeds. Guoan zheng, assistant professor biomedical engineering. Fourier ptychography is a computational imaging technique based on optical microscopy that consists in the synthesis of a wider numerical aperture from a set of full field images acquired at various coherent illumination angles, resulting in increased resolution compared to a conventional microscope. We report a lowcost, wide field, highresolution fourier ptychographic microscope fpm 14, implemented with 3dprinted optomechanics and a raspberry pi singleboard computer for data. Guoan zheng, roarke horstmeyer and changhuei yang, widefield, high resolution fourier ptychographic microscopy. Resolutionenhanced fourier ptychographic microscopy based on. We call this computational approach fourier ptychographic microscopy fpm. Counting wbcs can be done either manually or automatically.
Dexin ye, guoan zheng, jingyu wang, zhiyu wang, shan qiao, jiangtao huangfu, and lixin ran, negative group velocity in the absence of absorption resonance, scientific reports, 3, 1628 20. Pdf widefield highresolution 3d microscopy with fourier. Wide field, highresolution fourier ptychographic microscopy fpm guoan zheng1, 2, xiaoze ou1, roarke horstmeyer1 and changhuei yang1 1electrical engineering, california institute of technology, pasadena, ca, 91125, usa. Aperture fourier ptychographic microscopy mafpm is proposed here based on fourier ptychography fp, a technique to achieve wide fov and high resolution using timesequential synthesis of a high na coherent illumination. However, the timeconsuming process of image acquisition greatly narrows its applications in dynamic imaging. The method, termed fourier ptychographic tomography fpt, first captures a sequence of intensityonly images of a sample under angularly varying illumination. Details of conventional fp techniques can be found in g. The name of fourier ptychography comes from a related imaging modality, ptychography. Convolutional neural networks cnns have gained tremendous success in solving complex inverse problems. Fourier ptychographic microscopy fpm is a newly developed superresolution technique, which employs angularly varying illuminations and a phase retrieval algorithm to surpass the diffraction limit of a low numerical aperture na objective lens. Adaptive and efficient fourier ptychographic microscopy.
We discuss how fourier plane imaging microscopy is an application of a remarkable property of the obtained fp images. First, the procedure applies fourier ptychographic microscopy fpm to retrieve the amplitude and phase of a sample, at a resolution that. Fourier ptychography is a computational imaging technique based on optical microscopy that consists in the synthesis of a wider numerical aperture from a set of fullfield images acquired at various coherent illumination angles, resulting in increased resolution compared to a conventional microscope. This paper presents a technique to image the complex index of refraction of a sample across three dimensions. Accelerated wirtinger flow for multiplexed fourier. In the version of this article originally published, the reported resolution for the. We report a computational labelfree 3d microscopy technique, termed fourier ptychographic diffraction tomography fpdt. Aperturescanning fourier ptychography for 3d refocusing. Wide field, highresolution fourier ptychographic microscopy guoan zheng, roarke horstmeyer, and changhuei yang electrical engineering, california institute of technology, pasadena, ca 91125, usa. However, the dataacquisition process is timeconsuming, which is a critical issue. Guoan zheng, roarke horstmeyer and changhuei yang, wide field, highresolution fourier ptychographic microscopy, nature photonics, 7, 739745 20. Fourier ptychographic microscopy fpm is a highresolution computational.
Osa deep learning approach for fourier ptychography microscopy. Wide field, highresolution fourier ptychographic microscopy article pdf available in nature photonics 79. Wide fieldofview fluorescence image deconvolution with. Guoan zheng, roarke horstmeyer, and changhuei yang electrical engineering, california institute of technology, pasadena, ca 91125, usa. We report a novel lensless onchip microscopy platform based on nearfield blind ptychographic modulation.
In this article, we report an imaging method, termed fourier ptychographic microscopy fpm, which iteratively stitches together a number of variably illuminated. Fourier ptychographic microscopy using wavelength multiplexing. Fourier ptychographic microscopy fpm has been developed to overcome this limitation by using anglevaried illumination to enhance the performance of. Laser fourier ptychographic microscopy is highly efficient and noiserobust. Wide field highresolution 3d microscopy with fourier ptychographic diffraction tomography. Wide field, highresolution fourier ptychographic microscopy. Fourier ptychographic microscopy is a simple and effective computational imaging method that improves the spatial bandwidth product of the system.
Recently, fourier ptychography fp has been demonstrated for resolution improvement in coherent microscopy settings. Aperturescanning fourier ptychography for 3d refocusing and super resolution macroscopic imaging siyuan dong,1,5 roarke horstmeyer,3,5 radhika shiradkar,1,5 kaikai guo,1 xiaoze ou,3. Aperturescanning fourier ptychography for 3d refocusing and superresolution macroscopic imaging siyuan dong,1,5 roarke horstmeyer,3,5 radhika shiradkar,1,5 kaikai guo,1 xiaoze ou,3 zichao bian,1 huolin xin,4 and guoan zheng1,2, 1biomedical engineering, university of connecticut, storrs, connecticut 06269, usa 2electrical and computer engineering, university of connecticut, storrs. Using a set of lowresolution images that are recorded under varying illumination angles, the goal is to computationally reconstruct highresolution phase and amplitude images. Apr 20, 2019 we report a computational 3d microscopy technique, termed fourier ptychographic diffraction tomography fpdt, that iteratively stitches together numerous variably illuminated, lowresolution images acquired with a lownumerical aperture na objective in 3d fourier space to create a wide fieldofview fov, highresolution, depthresolved complex refractive index ri image across large. Fourier ptychographic microscopy fpm is a newly developed computational imaging technique that can provide gigapixel images with both high resolution hr and wide field of view fov. By blindly scanning the unknown diffuser to different xy positions, we acquire a.
Deep learning approach for fourier ptychography microscopy. So far, in many algorithms that have been proposed to solve the fpm reconstruction problem, the pupil function is set to be a fixed value such as the coherent transfer. The aim of this work is to develop a novel cnn framework to reconstruct video sequences of dynamic live cells captured using a computational microscopy technique, fourier ptychographic microscopy fpm. May 25, 2017 the present technology pertains generally to optical imaging and more particularly to a brightfield microscope with a programmable led light source, camera and processor for fourier ptychographic microscopy with multiplexed illumination for fast, wide field of view, high resolution imaging. A system and methods for wide field of view, high resolution fourier ptychographic microscopic imaging with a programmable led array light source. Wide field, highresolution fourier ptychographic microscopy skip to main content thank you for visiting. White blood cell wbc count is a valuable metric for assisting with diagnosis or prognosis of various diseases such as coronary heart disease, type 2 diabetes, or infection. Wide field, highresolution fourier ptychographic microscopy fpm guoan zheng1, 2, xiaoze ou1, roarke horstmeyer1 and changhuei yang1 1electrical engineering, california institute of technology, pasadena, ca, 91125, usa 2biomedical engineering and electrical engineering, university of connecticut, storrs, connecticut, 06269, usa wide fieldofview. It has applications in wide field, high resolution microscopy 1, microscopy biomedical imaging 2, long. Abstract it is inherently difficult for a conventional microscopic imaging system to produce images with both wide field of view fov and highresolution because of its limited space bandwidth product. Its principle is to realize the aperture expansion by using a phase retrieval algorithm to splice a set of lowresolution images under different illumination angles in the fourier domain. In this platform, we place a thin diffuser in between the object and the image sensor for light wave modulation. Structured illumination fluorescence fourier ptychographic.
Yang, wide field, highresolution fourier ptychographic microscopy, nature photonics 20 and in u. Sep 01, 20 in this article, we report an imaging method, termed fourier ptychographic microscopy fpm, which iteratively stitches together a number of variably illuminated, lowresolution intensity images in fourier space to produce a wide field, highresolution complex sample image. The resolution of final fp reconstruction is determined by the latest incident angle of the led array. This site is like a library, use search box in the widget to get ebook that you want. By adopting a wavefront correction strategy, the fpm method can also correct for aberrations and digitally extend a. Fourier ptychographic microscopy fpm is a high resolution computational imaging technique that is robust to recover high resolution image and large sbp by anglevaried illumination 4,5,6. The first one is the position misalignment of the led array, which can largely be. Aperturescanning fourier ptychography for 3d refocusing and. Guoan zheng, roarke horstmeyer and changhuei yang, widefield, highresolution fourier ptychographic microscopy.
Fourier ptychographic fp microscopy is a coherent imaging method that can synthesize an image with a higher bandwidth using multiple lowbandwidth images captured at different spatial frequency regions. Analysis of fourier ptychographic microscopy with half of the. Compressedsamplingbased fourier ptychographic microscopy. However, there are two possible reasons for position misalignment, which induce a degradation of the reconstructed image. The configuration is used in the scanning transmission electron microscope stem, and often in highresolution xray ptychography. Supplementary note 3 has been updated to reflect the corrigendum to the article. Here, we demonstrate for the first time that structured illumination fluorescence fourier ptychographic microscopy unites the advantages of fpm and sim. Fourier ptychographic adaptive denoising difference matrix data redundancy a b s t r a c t fourier ptychographic microscopy fpm is a recently developed wide field and highresolution hr imaging technique, reconstructing hr spectrum from a series of lowresolution lr images at different illumination angles. Pdf widefield, highresolution fourier ptychographic. Fourier ptychographic imaging download ebook pdf, epub. Fourier ptychographic microscopy fpm is a recently developed technique stitching lowresolution images in fourier domain to realize wide field highresolution imaging. Tunableillumination for laser fourier ptychographic.
Fourier ptychographic microscopy enables gigapixelscale imaging, with both large fieldofview and high resolution. Adaptive denoising method for fourier ptychographic microscopy. Osa widefield fourier ptychographic microscopy using laser. Efficient positional misalignment correction method for. Widefield highresolution 3d microscopy with fourier. Conventional light field microscope lacks the ability of high lateral resolution because it. Fpdt uses intensityonly measurements to create wide fieldofview, highresolution, depthresolved complex 3d refractive index images without resorting to interferometry. A simply equipped fourier ptychography platform based on.
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